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Polymeric Nanotraps for Immobilization and Delivery of Biomacromolecules
Coordinator/Contact person: Cor. Member Christo Tsvetanov DSc
Tel: +359 2 9792261
e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Funded by: NSFB
Grant agreement: DO 02-247/18.12.2008
Duration: 2009 - 2011
Partners: Institute of Molecular Biology - BAS
Abstract: The proposal meets the areas of chemical synthesis and properties of hollow nanoparticles together with encapsulation of biomacromolecules. The proposed research is in the new and exciting field now recognized as bionanotechnology. Bionanotechnology has already shown its great promise as an enabling technology in detection, imaging, and targeted drug delivery, and yet application of nano-materials to biological research still has not shown its full potential. The stability of biomacromolecules and their assemblies in solution is limited by degradation and denaturation. The main objective of the current proposal is to protect and to keep biomacromolecules in a fully functional state during storage and until safely delivered to a specific biological site. A major problem preventing the progression in encapsulation of biomacromolecules such as enzymes, proteins, nucleic acids into nano-containers is the issue of their stability during encapsulation, storage and release. Hollow polymeric nano- and microcapsules may hold a significant potential as bioreactors, drug delivery vehicles and DNA nanotraps. We aim to synthesize core-shell nano- and micro-particles comprising core of thermo-responsive polymers or copolymers as biomacromolecules engulfing templates, followed by coating with cross-linked hydrophilic protective shell. The form, the size and the functionality of the composite particles can be engineered with the aid of physical factors and chemical reactions. The templates will be removed from nanoparticles by dissolution at low temperature. We will try to encapsulate pDNA as well as other biomacromolecules such as enzymes and proteins. Important priority over other methods is that the operations will be performed entirely in aqueous medium in a very mild and non-destructive fashion.
Tel: +359 2 9792261
e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Funded by: NSFB
Grant agreement: DO 02-247/18.12.2008
Duration: 2009 - 2011
Partners: Institute of Molecular Biology - BAS
Abstract: The proposal meets the areas of chemical synthesis and properties of hollow nanoparticles together with encapsulation of biomacromolecules. The proposed research is in the new and exciting field now recognized as bionanotechnology. Bionanotechnology has already shown its great promise as an enabling technology in detection, imaging, and targeted drug delivery, and yet application of nano-materials to biological research still has not shown its full potential. The stability of biomacromolecules and their assemblies in solution is limited by degradation and denaturation. The main objective of the current proposal is to protect and to keep biomacromolecules in a fully functional state during storage and until safely delivered to a specific biological site. A major problem preventing the progression in encapsulation of biomacromolecules such as enzymes, proteins, nucleic acids into nano-containers is the issue of their stability during encapsulation, storage and release. Hollow polymeric nano- and microcapsules may hold a significant potential as bioreactors, drug delivery vehicles and DNA nanotraps. We aim to synthesize core-shell nano- and micro-particles comprising core of thermo-responsive polymers or copolymers as biomacromolecules engulfing templates, followed by coating with cross-linked hydrophilic protective shell. The form, the size and the functionality of the composite particles can be engineered with the aid of physical factors and chemical reactions. The templates will be removed from nanoparticles by dissolution at low temperature. We will try to encapsulate pDNA as well as other biomacromolecules such as enzymes and proteins. Important priority over other methods is that the operations will be performed entirely in aqueous medium in a very mild and non-destructive fashion.
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